CCL14 anticorps (Biotin)
Aperçu rapide pour CCL14 anticorps (Biotin) (ABIN116037)
Antigène
Voir toutes CCL14 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody reacts with Human Hemofiltrate CC Chemokine 1 (HCC-1).
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Purification
- Affinity chromatography.
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Immunogène
- Highly pure (>98%) recombinant human HCC-1.
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Indications d'application
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ELISA: To detect hHCC-1 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hHCC-1. Western Blot: To detect hHCC-1 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hHCC-1 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CCL14 (Chemokine (C-C Motif) Ligand 14 (CCL14))
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Autre désignation
- NCC2 / CCL14
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Sujet
- The CC cytokines are secreted proteins characterized by two adjacent cysteines. The cytokine encoded by this gene induces changes in intracellular calcium concentration and enzyme release in monocytes. This gene expresses both monocistronic and bicistronic transcripts. Bicistronic transcripts include the upstream cytokine gene CCL15. In addition, CCL14 undergoes alternative splicing in the bicistronic transcripts, it is unknown if its monocistronic transcript is also subject to alternative splicing.Synonyms: C-C motif chemokine 14, Chemokine CC-1/CC-3, HCC-1/HCC-3, NCC-2, SCYA14, Small-inducible cytokine A14
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ID gène
- 6358
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NCBI Accession
- NP_116738
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UniProt
- Q16627
Antigène
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