Interleukin 17a anticorps (Biotin)

N° du produit ABIN116117

L’anticorps Lapin Polyclonal anti-Interleukin 17a a été validé pour WB et EIA. Il convient pour détecter Interleukin 17a dans des échantillons de Humain.

Aperçu rapide pour Interleukin 17a anticorps (Biotin) (ABIN116117)

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Interleukin 17a est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Interleukin 17a anticorps (Biotin)

Specificité: Human Interleukin-17

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hIL-17A (human Interleukin 17A).

Information d'application

Indications d'application: ELISA: To detect hIL-17A by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hIL-17A. Western Blot: To detect hIL-17A by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-17A is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Shelf live: one year from despatch. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody at 4-8 °C for one month or at -20 °C for longer.

Détails sur Interleukin 17a

Antigène: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Autre désignation: Interleukin-17A (IL17A)

Sujet: IL17, also known as CTLA8, is a T cell derived hematopoietic cytokine. It was originally cloned from a T cell hybridoma produced by fusion of a mouse cytotoxic T cell clone and a rat T lymphoma. IL17 exhibits multiple biological activities on a variety of cells including: the induction of IL6, IL8 and GCSF production in fibroblasts, the enhancement of surface expression of ICAM 1 in fibroblasts, activation of NF kappaB and costimulation of T cell proliferation. IL17 is an approximately 16 kDa polypeptide of 136 amino acids. The precursor form of IL17 consists of 155 amino acids. To generate the mature IL17 (136 amino acids), the precursor cleaves a 19 amino acid signal peptide. Human IL17 shows approximately 62.5 % amino acid homology to mouse IL17 and 58 % amino acid homology to rat IL17.Synonyms: CTLA-8, CTLA8, Cytotoxic T-lymphocyte-associated antigen 8, IL-17, IL-17A, IL17

ID gène: 3605

NCBI Accession: NP_002181

UniProt: Q16552