CCL4 anticorps (Biotin)

N° du produit ABIN116165

L’anticorps Lapin Polyclonal anti-CCL4 a été validé pour WB et EIA. Il convient pour détecter CCL4 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour CCL4 anticorps (Biotin) (ABIN116165)

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL4 est conjugé à/à la Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-CCL4 anticorps (Biotin)

Specificité: Human Macrophage Inflammatory Protein-1 beta (hMIP-1b).

Purification: Affinity chromatography.

Immunogène: Highly pure (>98%) recombinant hMIP-1 beta (human MIP-1 beta).

Information d'application

Indications d'application: ELISA: To detect hMIP-1 beta by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthMIP-1 beta. Western Blot: To detect hMIP-1 beta by Western Blot analysis this antibody can be used ata concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMIP-1 beta is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C for one year. Following reconstitution the antibody can be stored at 2-8 °C for 2 weeks.

Date de péremption: 12 months

Références pour anticorps anti-CCL4 (Biotin) (ABIN116165)

Schlaepfer, Audigé, Joller, Speck: "TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).

Détails sur CCL4

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Autre désignation: MIP1 beta / CCL4

Sujet: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.Synonyms: CCL-4, G-26 T-lymphocyte-secreted protein, HC21, LAG1, Lymphocyte activation gene 1 protein, MIP-1-beta, MIP1B, Macrophage inflammatory protein 1-beta, PAT744, Protein H400, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2

ID gène: 388372

NCBI Accession: NP_001001435

UniProt: P13236