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CCL4 anticorps (Biotin)

CCL4 Reactivité: Humain WB, EIA Hôte: Lapin Polyclonal Biotin
N° du produit ABIN116165
  • Antigène Voir toutes CCL4 Anticorps
    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
    Reactivité
    • 57
    • 41
    • 17
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 88
    • 7
    • 2
    • 1
    • 1
    • 1
    • 1
    Lapin
    Clonalité
    • 92
    • 8
    Polyclonal
    Conjugué
    • 48
    • 16
    • 12
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CCL4 est conjugé à/à la Biotin
    Application
    • 86
    • 48
    • 33
    • 15
    • 13
    • 13
    • 13
    • 13
    • 9
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificité
    Human Macrophage Inflammatory Protein-1 beta (hMIP-1b).
    Purification
    Affinity chromatography.
    Immunogène
    Highly pure (>98%) recombinant hMIP-1 beta (human MIP-1 beta).
    Top Product
    Discover our top product CCL4 Anticorps primaire
  • Indications d'application
    ELISA: To detect hMIP-1 beta by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthMIP-1 beta. Western Blot: To detect hMIP-1 beta by Western Blot analysis this antibody can be used ata concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMIP-1 beta is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
    Buffer
    PBS, pH 7.2 without preservatives.
    Agent conservateur
    Without preservative
    Conseil sur la manipulation
    Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
    Stock
    4 °C/-20 °C
    Stockage commentaire
    Store the antibody prior to reconstitution at -20 °C for one year. Following reconstitution the antibody can be stored at 2-8 °C for 2 weeks.
    Date de péremption
    12 months
  • Schlaepfer, Audigé, Joller, Speck: "TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).

  • Antigène
    CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))
    Autre désignation
    MIP1 beta / CCL4 (CCL4 Produits)
    Synonymes
    anticorps ACT2, anticorps AT744.1, anticorps G-26, anticorps HC21, anticorps LAG-1, anticorps LAG1, anticorps MIP-1-beta, anticorps MIP1B, anticorps MIP1B1, anticorps SCYA2, anticorps SCYA4, anticorps CCL4, anticorps K203, anticorps Mip1-b, anticorps Scya4, anticorps MIP-1beta, anticorps MIP-1BETA, anticorps MIP1b, anticorps SCY4A, anticorps Act-2, anticorps MIP-1B, anticorps Mip1b, anticorps ACT-2, anticorps AT744.2, anticorps CCL4L, anticorps SCYA4L, anticorps SCYA4L1, anticorps SCYA4L2, anticorps C-C motif chemokine ligand 4, anticorps chemokine (C-C motif) ligand 4, anticorps chemokine (C-C motif) ligand 4-like 1, anticorps C-C motif chemokine ligand 4 like 1, anticorps CCL4, anticorps Ccl4, anticorps CCL4L1
    Sujet
    MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.Synonyms: CCL-4, G-26 T-lymphocyte-secreted protein, HC21, LAG1, Lymphocyte activation gene 1 protein, MIP-1-beta, MIP1B, Macrophage inflammatory protein 1-beta, PAT744, Protein H400, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2
    ID gène
    388372
    NCBI Accession
    NP_001001435
    UniProt
    P13236
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