CCL4 anticorps

N° du produit ABIN116168

Détail du produit anti-CCL4 anticorps

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificité: Recognizes Human Macrophage Inflammatory Protein-1 beta (MIP-1b). Other species not tested.

Purification: Affinity Chromatography employing immobilized Human MIP-1beta matrix.

Immunogène: Highly pure (>98%) E.coli derived recombinant Human MIP-1 beta (Cat.-No PA107)

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhMIP-1 beta (50.00 ng/mL), a concentration of 1.75-3.00 μg/mL of this antibody is required. ELISA: To detect hMIP-1 beta by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hMIP-1 beta. Western Blot: To detect hMIP-1 beta by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMIP-1 beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Références pour anticorps anti-CCL4 (ABIN116168)

Schlaepfer, Audigé, Joller, Speck: "TLR7/8 triggering exerts opposing effects in acute versus latent HIV infection." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 5, pp. 2888-95, (2006) (PubMed).

Détails sur CCL4

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Autre désignation: MIP1 beta / CCL4 (CCL4 Produits)

Synonymes: anticorps ACT2, anticorps AT744.1, anticorps G-26, anticorps HC21, anticorps LAG-1, anticorps LAG1, anticorps MIP-1-beta, anticorps MIP1B, anticorps MIP1B1, anticorps SCYA2, anticorps SCYA4, anticorps CCL4, anticorps K203, anticorps Mip1-b, anticorps Scya4, anticorps MIP-1beta, anticorps MIP-1BETA, anticorps MIP1b, anticorps SCY4A, anticorps Act-2, anticorps MIP-1B, anticorps Mip1b, anticorps ACT-2, anticorps AT744.2, anticorps CCL4L, anticorps SCYA4L, anticorps SCYA4L1, anticorps SCYA4L2, anticorps C-C motif chemokine ligand 4, anticorps chemokine (C-C motif) ligand 4, anticorps chemokine (C-C motif) ligand 4-like 1, anticorps C-C motif chemokine ligand 4 like 1, anticorps CCL4, anticorps Ccl4, anticorps CCL4L1

Sujet: MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94 % - 98 % identical and these proteins are all approximately 75 % homologous to murine MIP1 beta. MIP1 beta also shares approximately 70 % amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.Synonyms: CCL-4, G-26 T-lymphocyte-secreted protein, HC21, LAG1, Lymphocyte activation gene 1 protein, MIP-1-beta, MIP1B, Macrophage inflammatory protein 1-beta, PAT744, Protein H400, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2

ID gène: 388372

NCBI Accession: NP_001001435

UniProt: P13236