Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhNAP-2 (50.0 ng/mL), a concentration of 1.75-3.0 μg/mL of this antibody is required. ELISA: To detect hNAP-2 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
Buffer
PBS, pH 7.2 without preservatives
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.Synonyms: C-X-C motif chemokine 7, CTAP3, Leukocyte-derived growth factor, Macrophage-derived growth factor, SCYB7, Small-inducible cytokine B7, TGB1, THBGB1