CXCL7 anticorps
Aperçu rapide pour CXCL7 anticorps (ABIN116188)
Antigène
Voir toutes CXCL7 (PPBP) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody reacts with Human Neutrophil Activating Protein-2 (hNAP-2).
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Purification
- Affinity Chromatography using an immobilized Human NAP-2 matrix
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Immunogène
- Highly pure (>98%) E.coli derived recombinant Human NAP-2.
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Indications d'application
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhNAP-2 (50.0 ng/mL), a concentration of 1.75-3.0 μg/mL of this antibody is required. ELISA: To detect hNAP-2 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof recombinant hNAP-2. Western Blot: To detect hNAP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hNAP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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- CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
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Autre désignation
- NAP2 / PPBP / CXCL7
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Sujet
- NAP2 is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and sythesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.Synonyms: C-X-C motif chemokine 7, CTAP3, Leukocyte-derived growth factor, Macrophage-derived growth factor, SCYB7, Small-inducible cytokine B7, TGB1, THBGB1
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ID gène
- 5473
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NCBI Accession
- NP_002695
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UniProt
- P02775
Antigène
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