alpha Defensin 1 anticorps

N° du produit ABIN116330

L’anticorps anti-alpha Defensin 1 Polyclonal Chèvre est utilisé pour la détection de alpha Defensin 1 dans des échantillons de Humain. Il a été validé pour WB, EIA et Func.

Aperçu rapide pour alpha Defensin 1 anticorps (ABIN116330)

Antigène: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp alpha Defensin 1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-alpha Defensin 1 anticorps

Specificité: The antibody recognizes Human NP-1.

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography employing immobilized hNP-1 matrix

Immunogène: Highly pure (>98%) recombinant Human NP-1

Information d'application

Indications d'application: ELISA. Indirect: To detect hNP-1 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well ofrecombinant hNP-1. Sandwich: To detect hNP-1 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith a biotinylated Anti-Human NP-1 (PP1112B) as a detection antibody, allows thedetection of at least 0.2-0.4 ng/well of recombinant hNP-1. Western Blot: To detect hNP-1 by Western Blot analysis this antibody can be used at a concentration of0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant hNP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition (ND50) of the biological activity of hNP-1 (100 ng/mL),a concentration of 0.40-0.60 μg/mL of this antibody has been used with previous lots.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antibody is stable for one year from despatch at -20 °C. The reconstituted antibody is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Date de péremption: 12 months

Détails sur alpha Defensin 1

Antigène: alpha Defensin 1 (DEFA1) (Defensin, alpha 1 (DEFA1))

Autre désignation: Defensin alpha 1

Sujet: Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30 % of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.Synonyms: DEF-1, DEF-3, DEF-A1, DEF-A2, DEF-A3, DEF1, DEF3, DEFA-1, DEFA-2, DEFA-3, DEFA1, DEFA2, DEFA3, Defensin alpha 1, Defensin alpha 3, HNP-1, HNP-3, HNP1, HNP3, HP-1, HP-3, HP1, HP3, MRS, Neutrophil defensin 1, Neutrophil defensin 3

ID gène: 1667

NCBI Accession: NP_001035965

UniProt: P59665

Pathways: Cellular Response to Molecule of Bacterial Origin