CCL25 anticorps
Aperçu rapide pour CCL25 anticorps (ABIN116335)
Antigène
Voir toutes CCL25 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Thymus Expressed Chemokine (TECK)
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Purification
- Affinity chromatography.
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Immunogène
- Highly pure (>98%) recombinant hTECK (human Thymus Expressed Chemokine).
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Indications d'application
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhTECK (100 ng/mL), a concentration of 10.0- 15.0 μg/mL of this antibody is required. ELISA: To detect hTECK by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hTECK. Western Blot: To detect hTECK by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hTECK is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Reconstitution
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
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Buffer
- PBS, pH 7.2 without preservatives.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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