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beta 2 Defensin anticorps

Cet anticorps anti-beta 2 Defensin est un anticorps Chèvre Polyclonal détectant beta 2 Defensin dans WB, EIA et Neut. Adapté pour Humain.
N° du produit ABIN116361

Aperçu rapide pour beta 2 Defensin anticorps (ABIN116361)

Antigène

Voir toutes beta 2 Defensin (BD-2) Anticorps
beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

Reactivité

  • 37
  • 31
  • 16
  • 5
  • 2
  • 2
  • 1
Humain

Hôte

  • 59
  • 14
  • 4
  • 2
Chèvre

Clonalité

  • 64
  • 14
Polyclonal

Conjugué

  • 28
  • 7
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
Cet anticorp beta 2 Defensin est non-conjugé

Application

  • 39
  • 39
  • 39
  • 17
  • 15
  • 14
  • 13
  • 12
  • 10
  • 4
  • 4
  • 1
Western Blotting (WB), Enzyme Immunoassay (EIA), Neutralization (Neut)
  • Specificité

    This antibody recognizes Human BD2 (Defensin beta 2). Other species not tested.

    Purification

    Immunoaffinity Chromatography.

    Immunogène

    Highly pure (>98%) E.coli derived recombinant Human BD-2 (hBD-2)
  • Indications d'application

    Indirect ELISA: To detect Human BD-2 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof at least 0.2-0.4 ng/well of recombinant hBD-2. Sandwich ELISA: To detect hBD-2 by sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with biotinylated Anti-Human BD-2 (PP1125B1 or PP1125B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-2. Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

    Buffer

    PBS, pH 7.2 without preservatives

    Agent conservateur

    Without preservative

    Conseil sur la manipulation

    Avoid repeated freezing and thawing.

    Stock

    4 °C/-20 °C

    Stockage commentaire

    The lyophilized antibody is stable at -20 °C for one year from date of despatch. The reconstituted solution can be stored at 2-8 °C for two weeks. Frozen aliquots of this solution are stable for six months when kept at -20 °C.

    Date de péremption

    12 months
  • Antigène

    beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))

    Autre désignation

    Defensin beta 2

    Sujet

    This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.Synonyms: BD-2, Beta-defensin 2, Beta-defensin 4A, DEFB102, DEFB2, DEFB4A, DEFB4B, Skin-antimicrobial peptide 1, hBD-2

    ID gène

    100289462

    NCBI Accession

    NP_001192195

    UniProt

    O15263
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