beta 2 Defensin anticorps
Aperçu rapide pour beta 2 Defensin anticorps (ABIN116361)
Antigène
Voir toutes beta 2 Defensin (BD-2) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
-
-
Specificité
- This antibody recognizes Human BD2 (Defensin beta 2). Other species not tested.
-
Purification
- Immunoaffinity Chromatography.
-
Immunogène
- Highly pure (>98%) E.coli derived recombinant Human BD-2 (hBD-2)
-
-
-
-
Indications d'application
-
Indirect ELISA: To detect Human BD-2 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof at least 0.2-0.4 ng/well of recombinant hBD-2. Sandwich ELISA: To detect hBD-2 by sandwich ELISA (using 100 μL/well antibody solution)a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with biotinylated Anti-Human BD-2 (PP1125B1 or PP1125B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBD-2. Western Blot: To detect hBD-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBD-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
-
-
-
Reconstitution
- Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
-
Buffer
- PBS, pH 7.2 without preservatives
-
Agent conservateur
- Without preservative
-
Conseil sur la manipulation
- Avoid repeated freezing and thawing.
-
Stock
- 4 °C/-20 °C
-
Stockage commentaire
- The lyophilized antibody is stable at -20 °C for one year from date of despatch. The reconstituted solution can be stored at 2-8 °C for two weeks. Frozen aliquots of this solution are stable for six months when kept at -20 °C.
-
Date de péremption
- 12 months
-
-
- beta 2 Defensin (BD-2) (Defensin beta 2 (BD-2))
-
Autre désignation
- Defensin beta 2
-
Sujet
- This antibiotic peptide is locally regulated by inflammation. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. hBD-2 is a antimicrobial peptide, which belongs to the distinct family of beta-defensins.Synonyms: BD-2, Beta-defensin 2, Beta-defensin 4A, DEFB102, DEFB2, DEFB4A, DEFB4B, Skin-antimicrobial peptide 1, hBD-2
-
ID gène
- 100289462
-
NCBI Accession
- NP_001192195
-
UniProt
- O15263
Antigène
-