CXCL16 anticorps

N° du produit ABIN116405

Détail du produit anti-CXCL16 anticorps

Antigène: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CXCL16 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody recognizes Human CXCL16. Other species not tested.

Purification: Affinity Chromatography employing immobilized hCXCL16 matrix

Immunogène: Highly pure (> 98%) E.coli derived recombinant Human CXCL16

Information d'application

Indications d'application: Indirect ELISA: To detect hCXCL16 by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, in conjunctionwith compatible secondary reagents, allows the detection of 0.2-0.4 ng/well ofrecombinant hCXCL16. Sandwich ELISA: To detect hCXCL16 by sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antibody, inconjunction with a biotinylated anti-Human CXCL16 (PP1204B1 or PP1204B2) as a detectionantibody, allows the detection of 0.2-0.4 ng/well of recombinant hCXCL16. Western Blot: To detect hCXCL16 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. The detection limit for recombinant hCXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: The antibody stained FFPE Sections of human colorectaladenocarcinoma tissue. An HRP-labelled polymer detection system was used with a DAB chromogen). Antigenretrieval is not required for these conditions. Working Dilution: 0.5 μg/mL, overnight incubation at 4 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives

Agent conservateur: Without preservative

Conseil sur la manipulation: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Références pour anticorps anti-CXCL16 (ABIN116405)

Gutwein, Abdel-Bakky, Schramme, Doberstein, Kämpfer-Kolb, Amann, Hauser, Obermüller, Bartel, Abdel-Aziz, El Sayed, Pfeilschifter: "CXCL16 is expressed in podocytes and acts as a scavenger receptor for oxidized low-density lipoprotein." dans: The American journal of pathology, Vol. 174, Issue 6, pp. 2061-72, (2009) (PubMed).

Détails sur CXCL16

Antigène: CXCL16 (Chemokine (C-X-C Motif) Ligand 16 (CXCL16))

Autre désignation: CXCL16 (CXCL16 Produits)

Synonymes: anticorps CXCL16, anticorps 0910001K24Rik, anticorps AV290116, anticorps BB024863, anticorps CXCL16v1, anticorps CXCL16v2, anticorps SR-PSOX, anticorps Zmynd15, anticorps b2b498Clo, anticorps CXCLG16, anticorps SRPSOX, anticorps C-X-C motif chemokine ligand 16, anticorps chemokine (C-X-C motif) ligand 16, anticorps CXCL16, anticorps Cxcl16

Sujet: CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. It is a type I membrane protein (Potential). Also exists as a soluble form. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.Synonyms: C-X-C motif chemokine 16, SCYB16, SR-PSOX, SRPSOX, Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein, Small-inducible cytokine B16, Transmembrane chemokine CXCL16

ID gène: 58191

NCBI Accession: NP_001094282

UniProt: Q9H2A7