TNFSF14 anticorps

N° du produit ABIN116433

Cet anticorps anti-TNFSF14 Polyclonal Lapin (ABIN116433) détecte spécifiquement TNFSF14 dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour TNFSF14 anticorps (ABIN116433)

Antigène: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TNFSF14 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-TNFSF14 anticorps

Purification: Affinity chromatography employing immobilized hLIGHT matrix.

Immunogène: Highly pure (>98%) recombinant hLIGHT

Information d'application

Indications d'application: Neutralization. ELISA: To detect hLIGHT by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hLIGHT. Western Blot: To detect hLIGHT by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLIGHT is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: 0.5 X PBS pH 7.4 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur TNFSF14

Antigène: TNFSF14 (Tumor Necrosis Factor (Ligand) Superfamily, Member 14 (TNFSF14))

Autre désignation: CD258 / LIGHT

Sujet: LIGHT is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes and monocytes. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells. LIGHT binds to three receptors, the herpes virus entry mediator type A receptor (HEVM), LTbR and a decoy receptor (DcR3).Synonyms: HVEM-L, HVEML, Herpesvirus entry mediator-ligand, TNFSF14, Tumor necrosis factor ligand superfamily member 14

ID gène: 8740

NCBI Accession: NP_003798

UniProt: O43557

Pathways: Cancer Immune Checkpoints