DEFB103A anticorps

N° du produit ABIN116505

Détail du produit anti-DEFB103A anticorps

Antigène: DEFB103A (Defensin, beta 103A (DEFB103A))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DEFB103A est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.

Purification: Affinity Chromatography employing immobilized Human BD-3 matrix

Immunogène: E.coli derived Recombinant Human Beta Defensin-3.

Information d'application

Indications d'application: Indirect ELISA: To detect Human BD-3 by indirect ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This Antibody inconjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-3. Sandwich ELISA: To detect Human BD-3 by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. Antibody PP1229P,in conjunction with biotinylated anti-Human BD-3 (PP1229B1 and PP1229B1), allows thedetection of at least 0.2-0.4 ng/well of recombinant Human BD-3. Western Blot: To detect Human BD-3 by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondaryreagents the detection limit for recombinant Human BD-3 is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Centrifuge vial prior to opening. Restore with sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur DEFB103A

Antigène: DEFB103A (Defensin, beta 103A (DEFB103A))

Autre désignation: Defensin beta 3 (DEFB103A Produits)

Synonymes: anticorps BD-3, anticorps DEFB-3, anticorps DEFB103, anticorps DEFB3, anticorps HBD3, anticorps HBP-3, anticorps HBP3, anticorps hBD-3, anticorps DEFB103B, anticorps defensin beta 103A, anticorps defensin beta 103B, anticorps defensin, beta 103A, anticorps beta-defensin 103, anticorps DEFB103A, anticorps DEFB103B, anticorps LOC694235, anticorps LOC100359096

Sujet: Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma. Beta Defensin 3 belongs to the beta-defensin family.Synonyms: BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3

ID gène: 414325

NCBI Accession: NP_001075020

UniProt: P81534

Pathways: Production of Molecular Mediator of Immune Response