Intestinal Alkaline Phosphatase anticorps

N° du produit ABIN116600

L’anticorps anti-Intestinal Alkaline Phosphatase Polyclonal Lapin est utilisé pour la détection de Intestinal Alkaline Phosphatase dans des échantillons de Humain. Il a été validé pour WB et EIA.

Aperçu rapide pour Intestinal Alkaline Phosphatase anticorps (ABIN116600)

Antigène: Intestinal Alkaline Phosphatase (ALPI)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Intestinal Alkaline Phosphatase est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Intestinal Alkaline Phosphatase anticorps

Specificité: This antibody detects human Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Alkaline phosphatase [human intestine].

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène: Alkaline Phosphatase [Human Intestine].

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA and most immunological methodsrequiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of AlkalinePhosphatase [Human Intestine] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 5,000 to 1: 20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassiumphosphate, 0.15 M Sodium chloride, pH 7.2 with 0.01 % (w/v) Sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Détails sur Intestinal Alkaline Phosphatase

Antigène: Intestinal Alkaline Phosphatase (ALPI)

Autre désignation: Alkaline Phosphatase / ALPI / IAP

Sujet: Synonyms: Intestinal alkaline phosphatase

ID gène: 248

NCBI Accession: NP_001622

UniProt: P09923