CA1 anticorps

N° du produit ABIN116653

Détail du produit anti-CA1 anticorps

Antigène: CA1 (Carbonic Anhydrase I (CA1))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CA1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Carbonic Anhydrase I [Human Erythrocytes]. Cross reactivity against Carbonic Anhydrase I from other tissues and species may occur but have not been specifically determined.

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène: Carbonic Anhydrase I [Human Erythrocytes]

Information d'application

Indications d'application: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Carbonic Anhydrase I [HumanErythrocytes] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 1,000 to1: 5,000 is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 100.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4, with 0.01 % sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Détails sur CA1

Antigène: CA1 (Carbonic Anhydrase I (CA1))

Autre désignation: Carbonic Anhydrase 1 (CA1 Produits)

Synonymes: anticorps CA-I, anticorps CAB, anticorps Car1, anticorps AW555628, anticorps Ca1, anticorps Car-1, anticorps BG:DS00941.1, anticorps CAH, anticorps CG7820, anticorps Dmel\\CG7820, anticorps Gh7, anticorps ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3, anticorps ATBCA1, anticorps ATSABP3, anticorps BETA CARBONIC ANHYDRASE 1, anticorps F4P13.5, anticorps F4P13_5, anticorps SABP3, anticorps SALICYLIC ACID-BINDING PROTEIN 3, anticorps carbonic anhydrase 1, anticorps CA1, anticorps LOC100135826, anticorps GB15888, anticorps carbonic anhydrase 1, anticorps Carbonic anhydrase 1, anticorps carbonic anhydrase I, anticorps carbonic anhydrase, anticorps CA1, anticorps Car1, anticorps CAH1, anticorps LOC100135826, anticorps LOC408827, anticorps Tsp_05114, anticorps LOC100153915, anticorps LOC100050192, anticorps Ca1

Sujet: Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.Synonyms: CA-I, CA1, CAB, Carbonate dehydratase I, Carbonic anhydrase B, Carbonic anhydrase I

ID gène: 759

NCBI Accession: NP_001122301

UniProt: P00915