CA2 anticorps

N° du produit ABIN116657

L’anticorps anti-CA2 Polyclonal Lapin est utilisé pour la détection de CA2 dans des échantillons de Humain. Il a été validé pour WB et EIA.

Aperçu rapide pour CA2 anticorps (ABIN116657)

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CA2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-CA2 anticorps

Specificité: This antibody detects human Carbonic anhydrase 2. Cross reactivity against Carbonic anhydrase 2 from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [human erythrocytes].

Purification: Prepared from monospecific antiserum by a delipidation and defibrination

Immunogène: Carbonic Anhydrase II from Human Erythrocytes. Genename: CA2

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA (1/115,000) and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of CarbonicAnhydrase II [Human Erythrocytes] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) R1364HRP and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 to 1: 5,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium phosphate, 0.12 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide, None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Prior to and following reconstitution store undiluted at 2-8 °C. DO NOT FREEZE! This antibody should be protected from light as the PE-labelled product is photosensitive.

Détails sur CA2

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Autre désignation: Carbonic Anhydrase 2

Sujet: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

ID gène: 760

NCBI Accession: NP_000058

UniProt: P00918