CA2 anticorps

N° du produit ABIN116661

Cet anticorps anti-CA2 Polyclonal Lapin (ABIN116661) détecte spécifiquement CA2 dans WB, IP et EIA. L’anticorps est réactif avec des échantillons de Humain et Boeuf (Vache).

Aperçu rapide pour CA2 anticorps (ABIN116661)

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Reactivité: Humain, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CA2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-CA2 anticorps

Specificité: This antibody detects Carbonic anhydrase 2. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [bovine erythrocytes].

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogène: Carbonic Anhydrase II [Bovine Erythrocytes]

Information d'application

Indications d'application: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Carbonic Anhydrase II [BovineErythrocytes] in a standard sandwich ELISA using Peroxidase conjugated affinity purifiedanti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 14,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by refractometry)

Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.

Détails sur CA2

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Autre désignation: Carbonic Anhydrase 2

Sujet: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

ID gène: 280740

NCBI Accession: NP_848667

UniProt: P00921