CA2 anticorps

N° du produit ABIN116661

Détail du produit anti-CA2 anticorps

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Reactivité: Humain, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CA2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificité: This antibody detects Carbonic anhydrase 2. Immunoelectrophoresis give a single precipitin arc against purified and partially purified Carbonic anhydrase 2 [bovine erythrocytes].

Purification: This product was prepared from monospecific antiserum by delipidation and defibrination.

Immunogène: Carbonic Anhydrase II [Bovine Erythrocytes]

Information d'application

Indications d'application: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayed against 1.0 μg of Carbonic Anhydrase II [BovineErythrocytes] in a standard sandwich ELISA using Peroxidase conjugated affinity purifiedanti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 14,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by refractometry)

Buffer: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2, 0.01 % (w/v) Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Restore with 2.0 ml of deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.

Détails sur CA2

Antigène: CA2 (Carbonic Anhydrase II (CA2))

Autre désignation: Carbonic Anhydrase 2 (CA2 Produits)

Synonymes: anticorps CG6906, anticorps CT21171, anticorps Dmel\\CG6906, anticorps CA-2, anticorps caII, anticorps car2, anticorps ca ii, anticorps ca-ii, anticorps BETA CA2, anticorps BETA CARBONIC ANHYDRASE 2, anticorps CA18, anticorps CARBONIC ANHYDRASE 18, anticorps CARBONIC ANHYDRASE 2, anticorps T9L3.40, anticorps T9L3_40, anticorps carbonic anhydrase 2, anticorps LOC100135827, anticorps AI131712, anticorps CAII, anticorps Ca2, anticorps Car-2, anticorps Ltw-5, anticorps Lvtw-5, anticorps CA-II, anticorps CAC, anticorps Car2, anticorps CAR3, anticorps Carbonic anhydrase 2, anticorps carbonic anhydrase 2 L homeolog, anticorps carbonic anhydrase 2, anticorps carbonic anhydrase 1, anticorps carbonic anhydrase II, anticorps carbonic anhydrase, alpha type, periplasmic, anticorps CAH2, anticorps ca2.L, anticorps ca2, anticorps CA2, anticorps HCAG_04637, anticorps LOC100135827, anticorps LOC100635510, anticorps Car2, anticorps Ca2, anticorps LOC100154873, anticorps LOC101113341

Sujet: Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29 kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20μM. It catalyses the hydration of CO2 and the hydrolysis of esters. Zn2+ ion cofactor is required for enzyme activity. Certain point mutations occur without apparent clinical effect, however, complete absence leads to mild mental retardation and cerebral calcification, osteoporosis and renal tubular acidosis.Synonyms: CA-II, CA2, CAC, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II

ID gène: 280740

NCBI Accession: NP_848667

UniProt: P00921