Catalase anticorps

N° du produit ABIN116677

Cet anticorps Lapin Polyclonal détecte spécifiquement Catalase dans WB, IP et EIA. Il présente une réactivité avec des échantillons de Boeuf (Vache).

Aperçu rapide pour Catalase anticorps (ABIN116677)

Antigène: Catalase (CAT)

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Catalase est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-Catalase anticorps

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Catalase [Bovine Liver]. Cross reactivity against Catalase from other tissues and species may occur but have not been specifically determined.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène: Catalase from Bovine liver.

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation,Conjugation and most immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Catalase [Bovineliver] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) (R1364HRP) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 26,000 to 1: 142,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 5.0 mL of deionized water (or equivalent).

Concentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 containing no preservative.

Agent conservateur: Without preservative

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Détails sur Catalase

Antigène: Catalase (CAT)

Autre désignation: Catalase

Sujet: Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.Synonyms: CAT, Peroxisome Marker

ID gène: 531682

NCBI Accession: NP_001030463

UniProt: P00432

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism