Pancreatic Islet Lysate anticorps

N° du produit ABIN116793

Cet anticorps anti-Pancreatic Islet Lysate Polyclonal Chien (ABIN116793) détecte spécifiquement Pancreatic Islet Lysate dans WB et EIA. L’anticorps est réactif avec des échantillons de Porc.

Aperçu rapide pour Pancreatic Islet Lysate anticorps (ABIN116793)

Antigène: Pancreatic Islet Lysate

Reactivité: Porc

Hôte: Chien

Clonalité: Polyclonal

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit

Purification: Multi-step process.

Immunogène: Sonicated crude membrane fraction of porcine pancreatic islets of langerhans cells.

Information d'application

Indications d'application: Suitable for Immunoblotting (Western or Dot blot) and ELISA. Recommended Dilutions: This product has been assayed against 1.0 μg of Pancreatic IsletLysate [Porcine Pancreas] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Dog IgG [H&L] (Goat) and o-Phenylenediamine (OPD) as a substrate. Aworking dilution of 1: 50 to 1: 100 is suggested for this product. This product has beenassayed against 1.5 μg of Pancreatic Islet Lysate [Porcine Pancreas] per lane byImmunoblot (Western) under reducing conditions of SDS-PAGE prior to transfer tonitrocellulose and detection using Peroxidase conjugated Affinity Purified anti-Dog IgG[H&L] (Goat) and Diaminobenzidine (DAB) as a substrate. A working dilution of 1: 50 to1: 200 is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: -20 °C

Stockage commentaire: Store vial at -70 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Pancreatic Islet Lysate