Pepsin anticorps
Aperçu rapide pour Pepsin anticorps (ABIN116802)
Antigène
Voir toutes Pepsin AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pepsin [Porcine stomach]. Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined.
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Purification
- Prepared from monospecific antiserum by a delipidation and defibrination.
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Immunogène
- Pepsin [Porcine stomach].
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Indications d'application
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostImmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Pepsin [Porcinestomach] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 3,000 to1: 11,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Restore with 2.0 mL of deionized water (or equivalent).
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Concentration
- 90.0 mg/mL (by Refractometry)
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Buffer
- 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 with 0.01 % (w/v) Sodium Azide as preservative.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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