Laminin anticorps (Biotin)

N° du produit ABIN116904

Cet anticorps anti-Laminin est un anticorps Lapin Polyclonal détectant Laminin dans WB, IP, IHC (p), IHC (fro) et EIA. Adapté pour Humain.

Aperçu rapide pour Laminin anticorps (Biotin) (ABIN116904)

Antigène: Laminin (LN)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Laminin est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Détail du produit anti-Laminin anticorps (Biotin)

Attributs du produit: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.

Purification: Immunoaffinity chromatography.

Immunogène: Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.

Information d'application

Indications d'application: Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against Laminin at a dilution of 1: 5,000 to 1: 10,000. This product was also assayedagainst 1.0 μg of Laminin in a standard capture ELISA using Peroxidase ConjugatedStreptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 4,000 to 1: 8,000 of the stockconcentration is suggested for this product. For Immunohistochemistry on paraffinembedded tissue dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody (in aliquots) at -20 °C.

Détails sur Laminin

Antigène: Laminin (LN)

Autre désignation: Laminin