Laminin anticorps (Biotin)
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- Antigène Voir toutes Laminin (LN) Anticorps
- Laminin (LN)
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Reactivité
- Humain
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Laminin est conjugé à/à la Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Attributs du produit
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
- Purification
- Immunoaffinity chromatography.
- Immunogène
- Produced against a mixture of synthetic peptides corresponding to regions of adherence on laminin.
- Top Product
- Discover our top product LN Anticorps primaire
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- Indications d'application
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Anti-Laminin antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against Laminin at a dilution of 1: 5,000 to 1: 10,000. This product was also assayedagainst 1.0 μg of Laminin in a standard capture ELISA using Peroxidase ConjugatedStreptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substratefor 30 minutes at room temperature. A working dilution of 1: 4,000 to 1: 8,000 of the stockconcentration is suggested for this product. For Immunohistochemistry on paraffinembedded tissue dilute the product 1: 50 to 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- -20 °C
- Stockage commentaire
- Store the antibody (in aliquots) at -20 °C.
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- Antigène
- Laminin (LN)
- Autre désignation
- Laminin (LN Produits)
- Synonymes
- anticorps LAMC1, anticorps laminin, anticorps laminin, beta 2 (laminin S), anticorps LAMB2
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