Fibronectin anticorps (Biotin)

N° du produit ABIN116907

Cet anticorps anti-Fibronectin est un anticorps Lapin Polyclonal détectant Fibronectin dans WB, IP, IHC (p), EIA et IHC (fro). Adapté pour Humain.

Aperçu rapide pour Fibronectin anticorps (Biotin) (ABIN116907)

Antigène: Fibronectin

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Fibronectin est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Fibronectin anticorps (Biotin)

Specificité: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Fibronectins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.

Attributs du produit: Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.

Purification: Immunoaffinity Chromatography.

Immunogène: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.

Information d'application

Indications d'application: Anti-Fibronectin antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for Western blottingfor highly sensitive qualitative analysis. This product was assayed by immunoblot and found to be reactive against Fibronectin at adilution of 1: 5,000 to 1: 10,000. This product was also assayed against 1.0 ?g of Fibronectin in a standard capture ELISAusing Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water or equivalent.

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below.

Détails sur Fibronectin

Antigène: Fibronectin

Sujet: Human fibronectin has a molecular weight of 450 kDa when purified in an intact form from plasma. Fibronectin is a glycoprotein synthesized in the liver for the circulating blood plasma form, and is synthesized by many mesenchymal cells, for the extracellular matrix form. It is composed of two similar, but not identical protein chains, which are linked by two disulfide linkages at the C-terminal end of the chains. The chains are composed of domains which have specific secondary structures linked together by regions which are especially susceptible to proteolytic action. For this reason, detection by immunoblot (western) may show considerable variability in the observed apparent molecular weights, which is predicated on the source of the fibronectin, and to what degree proteolysis has occurred. Bands approximately 225 kDa shoeld be observed after SDS-PAGE when reducing and denaturing conditions are used.Synonyms: CIG, Cold-insoluble globulin, FN1

ID gène: 2335

NCBI Accession: NP_002017

UniProt: P02751