Recombinant Rab1-GTP anticorps

N° du produit ABIN1169138

Détail du produit

Antigène: Rab1-GTP

Type d'anticorp: Recombinant Antibody

Reactivité: Chien, Humain, Souris, Rat

Hôte: Human

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificité: Recognizes human, mouse, rat and dog Rab1a-GTP and Rab1b-GTP.

Réactivité croisée: Chien, Humain, Souris, Rat (Rattus)

Purification: Purified from HEK 293 cell culture supernatant.

Pureté: >95 % (SDS-PAGE)

Immunogène: Full length canine Rab1.

Clone: ROF7

Isotype: IgG2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Référence

Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." dans: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

Détail du antigène

Antigène: Rab1-GTP

Sujet: Rab1 (Ypt1 in yeast) is a small GTPase that plays a well-established role in mediating ER-to-Golgi protein transport in both yeast and mammalian cells. Rab1 recruits effector proteins to budding COPII vesicles at the ER, forming cis-SNARE complexes that promote targeting to and fusion of these vesicles with the cis-Golgi. Rab1 is also involved in COPI vesicle formation and other distinct transport pathways, including ER-to-Golgi intermediate compartment (ERGIC)-to-cell periphery trafficking. Recently Rab1 has been shown to function in antimicrobial autophagy (autophagosome formation), as well as other forms of autophagy in mammalian cells in a way independent of ER-to-Golgi trafficking. anti-Rab1-GTP, monoclonal antibody (recombinant) (ROF7) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

UniProt: P62822