Polyglutamylation anticorps (Biotin)

N° du produit ABIN1169177

Détail du produit

Antigène: Polyglutamylation

Reactivité: Toutes les espèces

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Biotin

Application: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)

Specificité: Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin.

Réactivité croisée: Toutes les espèces

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Pureté: >95 % (SDS-PAGE)

Immunogène: Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).

Clone: GT335

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Recognizes most forms of polyglutamylated tubulin (alpha- and beta-tubulin), independent of the length of the glutamate side chains. No specificity to particular tubulin isoforms nor to tubulin from particular species are observed. Detects also other (poly)glutamylated proteins. Since no consensus modification site is known for protein (poly)glutamylation, the detection is not sequence-specific. However, an acidic environment of the modification site is required. The use of the antibody at too high concentrations obscures its specificity in immunofluorescence.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détail du antigène

Antigène: Polyglutamylation

Sujet: Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and be involved in cell cycle and cell proliferation.