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IL-10 anticorps (AA 19-178) (Biotin)

Cet anticorps anti-IL-10 est un anticorps Lapin Polyclonal détectant IL-10 dans WB, IHC et ICC. Adapté pour Souris.
N° du produit ABIN1172112

Aperçu rapide pour IL-10 anticorps (AA 19-178) (Biotin) (ABIN1172112)

Antigène

Voir toutes IL-10 (IL10) Anticorps
IL-10 (IL10) (Interleukin 10 (IL10))

Reactivité

  • 167
  • 100
  • 55
  • 22
  • 21
  • 12
  • 10
  • 8
  • 8
  • 8
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  • 7
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  • 1
  • 1
  • 1
Souris

Hôte

  • 146
  • 87
  • 80
  • 4
  • 2
  • 2
  • 2
Lapin

Clonalité

  • 182
  • 138
  • 1
Polyclonal

Conjugué

  • 188
  • 38
  • 18
  • 14
  • 12
  • 7
  • 3
  • 2
  • 2
  • 2
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  • 2
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  • 2
  • 2
  • 2
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  • 2
  • 2
  • 2
  • 2
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  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
Cet anticorp IL-10 est conjugé à/à la Biotin

Application

  • 186
  • 139
  • 107
  • 76
  • 64
  • 51
  • 30
  • 17
  • 16
  • 16
  • 15
  • 14
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  • 1
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  • 1
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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Épitope

    • 51
    • 16
    • 16
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
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    • 1
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    • 1
    AA 19-178

    Fonction

    Biotin-Linked Polyclonal Antibody to Interleukin 10 (IL10)

    Séquence

    MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- SR GQYSREDNNC THFPVGQSHM LLELRTAFSQ VKTFFQTKDQ LDNILLTDSL MQDFKGYLGC QALSEMIQFY LVEVMPQAEK HGPEIKEHLN SLGEKLKTLR MRLRRCHRFL KCENKSKAVE QVKSDFNKLE DQGVYKAMNE FDIFINCIEA YMMIKMKS

    Specificité

    The antibody is a rabbit polyclonal antibody raised against IL10. It has been selected for its ability to recognize IL10 in immunohistochemical staining and western blotting.

     Réactivité croisée

    Rat

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    Recombinant IL10 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against IL10 conjugated to biotin.

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    IL-10 (IL10) (Interleukin 10 (IL10))

    Autre désignation

    Interleukin 10

    Sujet

    CSIF, IL10A, TGIF, Cytokine Synthesis Inhibitory Factor

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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