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CA 19-9 anticorps (AA 35-361) (Biotin)

L’anticorps Lapin Polyclonal anti-CA 19-9 a été validé pour IHC, ELISA et WB. Il convient pour détecter CA 19-9 dans des échantillons de Humain.
N° du produit ABIN1172465

Aperçu rapide pour CA 19-9 anticorps (AA 35-361) (Biotin) (ABIN1172465)

Antigène

Voir toutes CA 19-9 Anticorps
CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

Reactivité

  • 42
  • 1
  • 1
Humain

Hôte

  • 28
  • 14
  • 2
Lapin

Clonalité

  • 42
  • 1
Polyclonal

Conjugué

  • 28
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp CA 19-9 est conjugé à/à la Biotin

Application

  • 25
  • 18
  • 16
  • 16
  • 8
  • 7
  • 6
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
  • Épitope

    • 1
    • 1
    AA 35-361

    Séquence

    MGHHHHHHSG SEF-RVSRDD ATGSPRAPSG SSRQDTTPTR PTLLILLWTW PFHIPVALSR CSEMVPGTAD CHITADRKVY PQADTVIVHH WDIMSNPKSR LPPSPRPQGQ RWIWFNLEPP PNCQHLEALD RYFNLTMSYR SDSDIFTPYG WLEPWSGQPA HPPLNLSAKT ELVAWAVSNW KPDSARVRYY QSLQAHLKVD VYGRSHKPLP KGTMMETLSR YKFYLAFENS LHPDYITEKL WRNALEAWAV PVVLGPSRSN YERFLPPDAF IHVDDFQSPK DLARYLQELD KDHARYLSYF RWRETLRPRS FSWALDFCKA CWKLQQESRY QTVRSIAAWF T

    Specificité

    It has been selected for its ability to recognize CA19-9 in immunohistochemical staining and Western blotting.

    Purification

    Affinity Chromatography

    Immunogène

    Recombinant CA19-9 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against CA19-9 conjugated to biotin.

    Isotype

    IgG
  • Indications d'application

    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.

    Commentaires

    Content: The quality control contains recombinant CA19-9 (Arg35~Thr361) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C

    Stockage commentaire

    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

    Date de péremption

    12 months
  • Antigène

    CA 19-9 (Gastrointestinal Cancer Antigen CA19-9 (CA 19-9))

    Autre désignation

    Carbohydrate Antigen 19-9 (CA19-9)
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