VWF anticorps (AA 1498-1665) (Biotin)

N° du produit ABIN1173732

Détail du produit anti-VWF anticorps (Biotin)

Antigène: VWF (Von Willebrand Factor (VWF))

Épitope: AA 1498-1665

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp VWF est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Séquence: MGHHHHHHSG SEF-DVV FVLEGSDEVG EANFNKSKEF VEEVIQRMDV SPDATRISVL QYSYTVTMEY AFNGAQSKEE VLRHVREIRY QGGNRTNTGQ ALQYLSEHSF SPSQGDRVEA PNLVYMVTGN PASDEIKRLP GDIQVVPIGV GPHANMQELE RISRPIAPIF IRDFETLPRE APDLV

Specificité: It has been selected for its ability to recognize vWF in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogène: Recombinant vWF expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against vWF conjugated to biotin.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Commentaires:

Content: The quality control contains recombinant vWF (Asp1498~Val1665) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Date de péremption: 12 months

Détails sur VWF

Antigène: VWF (Von Willebrand Factor (VWF))

Abstract: VWF Produits

Synonymes: anticorps VWF, anticorps si:ch1073-474e24.1, anticorps F8VWF, anticorps VWD, anticorps 6820430P06Rik, anticorps AI551257, anticorps B130011O06Rik, anticorps C630030D09, anticorps von Willebrand factor, anticorps Von Willebrand factor, anticorps VWF, anticorps vwf, anticorps Vwf