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Ki-67 anticorps (AA 2815-2928) (Biotin)

Cet anticorps Lapin Polyclonal détecte spécifiquement Ki-67 dans IHC, WB et ICC. Il présente une réactivité envers Humain.
N° du produit ABIN1175455

Aperçu rapide pour Ki-67 anticorps (AA 2815-2928) (Biotin) (ABIN1175455)

Antigène

Voir toutes Ki-67 (MKI67) Anticorps
Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivité

  • 167
  • 47
  • 28
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
Humain

Hôte

  • 92
  • 71
  • 12
  • 3
  • 1
Lapin

Clonalité

  • 98
  • 79
Polyclonal

Conjugué

  • 100
  • 11
  • 11
  • 5
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp Ki-67 est conjugé à/à la Biotin

Application

  • 100
  • 90
  • 72
  • 49
  • 40
  • 31
  • 21
  • 19
  • 18
  • 16
  • 13
  • 6
  • 6
  • 5
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)
  • Épitope

    • 30
    • 16
    • 7
    • 7
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2815-2928

    Fonction

    Biotin-Linked Polyclonal Antibody to Ki-67 Protein (Ki-67)

    Séquence

    MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- MTDDKT TKIPCKSSPE LEDTATSSKR RPRTRAQKVE VKEELLAVGK LTQTSGETTH TDKEPVGEGK GTKAFKQPAK RKLDAEDVIG SRRQPRAPKE KAQPLEDLAS FQELSQTP

    Specificité

    The antibody is a rabbit polyclonal antibody raised against Ki-67. It has been selected for its ability to recognize Ki-67 in immunohistochemical staining and western blotting.

     Réactivité croisée

    Souris

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    Recombinant Ki67P expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against Ki67P conjugated to biotin.

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Antigène

    Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

    Autre désignation

    Ki-67 Protein

    Sujet

    MKI67, KIA, Ki67P, Antigen Identified By Monoclonal Antibody Ki-67

    Pathways

    Glycosaminoglycan Metabolic Process
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