KLRG1 anticorps

N° du produit ABIN1176845

Détail du produit anti-KLRG1 anticorps

Antigène: KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))

Reactivité: Souris

Hôte: Golden Syrian Hamster

Clonalité: Monoclonal

Conjugué: Cet anticorp KLRG1 est non-conjugé

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: A-LAK from C57BL/6 mice

Clone: 2F1

Isotype: IgG2 kappa

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-KLRG1 (ABIN1176845)

Beyersdorf, Ding, Karp, Hanke: "Expression of inhibitory "killer cell lectin-like receptor G1" identifies unique subpopulations of effector and memory CD8 T cells." dans: European journal of immunology, Vol. 31, Issue 12, pp. 3443-52, (2001) (PubMed).

Corral, Hanke, Vance, Cado, Raulet: "NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules." dans: European journal of immunology, Vol. 30, Issue 3, pp. 920-30, (2000) (PubMed).

Hanke, Corral, Vance, Raulet: "2F1 antigen, the mouse homolog of the rat "mast cell function-associated antigen", is a lectin-like type II transmembrane receptor expressed by natural killer cells." dans: European journal of immunology, Vol. 28, Issue 12, pp. 4409-17, (1999) (PubMed).

Détails sur KLRG1

Antigène: KLRG1 (Killer Cell Lectin-Like Receptor Subfamily G, Member 1 (KLRG1))

Autre désignation: KLRG1 (KLRG1 Produits)

Synonymes: anticorps KLRG1, anticorps 2F1-Ag, anticorps MAFA, anticorps MAFA-L, anticorps 2F1, anticorps CLEC15A, anticorps MAFA-2F1, anticorps MAFA-LIKE, anticorps killer cell lectin like receptor G1, anticorps killer cell lectin-like receptor subfamily G, member 1, anticorps KLRG1, anticorps Klrg1

Sujet: The 2F1 monoclonal antibody specifically binds to KLRG1 (Killer cell Lectin-like Receptor G1), which is the mouse homologue of the rat mast cell function-associated antigen (MAFA), on all mouse strains tested (eg, AKR/J, BALB/c, C3H/HeN, C3H.SW, C57BL/6, DBA/1, SJL, 129/J). Unlike rat MAFA, which is expressed on mast cells, mouse KLRG1 is expressed on a large subset of NK cells, lymphokine-activated killer (LAK) cells, adherent LAK (A-LAK) cells, subsets of activated CD8+ T lymphocytes, and small fractions of CD4+ and CD8+ T cells, but not mast cells. The expression of KLRG1 is correlated with reduced proliferative capacity of activated T lymphocytes or reduced effector functions of activated NK cells. This molecule is believed to play a common role in the regulation of leukocytes of both the innate and adaptive immune system. It has been observed that the 2F1 mAb stains the rat basophilic leukemia cell line, RBL-2H3, which is known to express MAFA. The KLRG1 protein is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif), its ligand has not been identified KLRG1 is expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kDa. The level of KLRG1 expression is reduced in MHC class I-deficient mice, although direct binding of KLRG1 to MHC class I antigens could not be detected. Cross-linking of KLRG1 by 2F1 mAb reduces TCR-mediated Ca++ mobilization and cytotoxic responses (but not IFN-gamma production) by CD8+ T cells and inhibits IFN-gamma and TNF-alpha production and redirected lysis by NK cells.
Synonyms: Klrg1, Killer cell lectin-like receptor subfamily G member 1, MAFA