B-Cell Linker anticorps (N-Term) (Alexa Fluor 647)

N° du produit ABIN1176912

Cet anticorps anti-B-Cell Linker est un anticorps Souris Monoclonal détectant B-Cell Linker dans ICS. Adapté pour Humain et Souris. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour B-Cell Linker anticorps (N-Term) (Alexa Fluor 647) (ABIN1176912)

Antigène: B-Cell Linker (BLNK)

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp B-Cell Linker est conjugé à/à la Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: 2B11

Détail du produit anti-B-Cell Linker anticorps (Alexa Fluor 647)

Épitope: N-Term

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human N-terminal BLNK Recombinant Protein

Isotype: IgG2a kappa

Information d'application

Indications d'application: This antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer). Any of the three BD Phosflow™ permeabilization buffers may be used.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Références pour anticorps anti-B-Cell Linker (Alexa Fluor 647) (ABIN1176912)

Taguchi, Kiyokawa, Takenouch, Matsui, Tang, Nakajima, Suzuki, Shiozawa, Saito, Katagiri, Takahashi, Karasuyama, Matsuo, Okita, Fujimoto: "Deficiency of BLNK hampers PLC-gamma2 phosphorylation and Ca2+ influx induced by the pre-B-cell receptor in human pre-B cells." dans: Immunology, Vol. 112, Issue 4, pp. 575-82, (2004) (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." dans: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, (2004) (PubMed).

Li, Martin, Oliver, Kearney, Carter: "Antigen receptor proximal signaling in splenic B-2 cell subsets." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 5, pp. 3122-9, (2001) (PubMed).

Minegishi, Rohrer, Coustan-Smith, Lederman, Pappu, Campana, Chan, Conley: "An essential role for BLNK in human B cell development." dans: Science (New York, N.Y.), Vol. 286, Issue 5446, pp. 1954-7, (1999) (PubMed).

Détails sur B-Cell Linker

Antigène: B-Cell Linker (BLNK)

Autre désignation: BLNK

Sujet: B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The 2B11 monoclonal antibody recognizes BLNK, regardless of phosphorylation status. A fusion protein representing amino acids 4-205 of human BLNK was used as the immunogen. BLNK is expressed as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE that represent alternatively spliced forms of human BLNK.
Synonyms: SLP-65, BASH, BCA

Pathways: BCR Signaling