ElF4E (eIF-4E, CBP, EIF4E) (pSer209) anticorps (Alexa Fluor 647)

N° du produit ABIN1177053

Cet anticorps anti- est un anticorps Souris Monoclonal détectant dans ICS. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour ElF4E (eIF-4E, CBP, EIF4E) (pSer209) anticorps (Alexa Fluor 647) (ABIN1177053)

Antigène: ElF4E (eIF-4E, CBP, EIF4E)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Alexa Fluor 647

Application: Intracellular Staining (ICS)

Clone: J77-925

Détail du produit

Épitope: pSer209

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human eIF4E Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Référence

Culjkovic, Topisirovic, Borden: "Controlling gene expression through RNA regulons: the role of the eukaryotic translation initiation factor eIF4E." dans: Cell cycle (Georgetown, Tex.), Vol. 6, Issue 1, pp. 65-9, (2007) (PubMed).

Wendel, Silva, Malina, Mills, Zhu, Ueda, Watanabe-Fukunaga, Fukunaga, Teruya-Feldstein, Pelletier, Lowe: "Dissecting eIF4E action in tumorigenesis." dans: Genes & development, Vol. 21, Issue 24, pp. 3232-7, (2007) (PubMed).

Grolleau, Kaplan, Hanash, Beretta, Richardson: "Impaired translational response and increased protein kinase PKR expression in T cells from lupus patients." dans: The Journal of clinical investigation, Vol. 106, Issue 12, pp. 1561-8, (2001) (PubMed).

Waskiewicz, Flynn, Proud, Cooper: "Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2." dans: The EMBO journal, Vol. 16, Issue 8, pp. 1909-20, (1997) (PubMed).

Détail du antigène

Antigène: ElF4E (eIF-4E, CBP, EIF4E)

Sujet: The eukaryotic translation Initiation Factor 4E (eIF4E) is a 25-kDa phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mRNA. eIF4E is the rate-limiting component for the initiation of cap-dependent translation by the eIF4F translation initiation complex, which is composed of eIF4E, eIF4G, and eIF4A. This complex promotes the unwinding of secondary structure at the 5' untranslated region of mRNA that is necessary to expose and locate the AUG initiation codon. Other functions of eIF4E have been identified, such as promoting the export of mRNAs that are involved in cell cycle progression from the nucleus and differentially regulating the translation of certain mRNAs in the cytoplasm. Three mechanisms for eIF4E regulation have been identified: Mnk1-mediated phosphorylation on serine 209 (S209) is required for eIF4E binding to the cap structure, over-expression of phosphorylated eIF4E can lead to increased cell proliferation, suppression of apoptosis, and a transformed phenotype, and interactions with nonphosphorylated eIF4E-binding proteins inhibit the formation of the eIF4F complex. The J77-925 monoclonal antibody recognizes the phosphorylated S209 (pS209) of eIF4E.
Synonyms: eIF-4E, CBP, EIF4E1, EIF4EL1, EIF4F, mRNA cap-binding protein