Ezrin anticorps (pTyr353) (Alexa Fluor 488)

N° du produit ABIN1177059

Cité dans 2 publications. Cet anticorps anti-Ezrin Monoclonal Souris conjugué à Alexa Fluor 488 (Clone I66-386) (ABIN1177059) détecte spécifiquement Ezrin dans ICS. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Ezrin anticorps (pTyr353) (Alexa Fluor 488) (ABIN1177059)

Antigène: Ezrin (EZR)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ezrin est conjugé à/à la Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: I66-386

Détail du produit anti-Ezrin anticorps (Alexa Fluor 488)

Épitope: pTyr353

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human Ezrin Peptide

Isotype: IgG1 kappa

Information d'application

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-Ezrin (Alexa Fluor 488) (ABIN1177059)

Gautreau, Poullet, Louvard, Arpin: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, (1999) (PubMed).

Krieg, Hunter: "Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin." dans: The Journal of biological chemistry, Vol. 267, Issue 27, pp. 19258-65, (1992) (PubMed).

Détails sur Ezrin

Antigène: Ezrin (EZR)

Autre désignation: Ezrin

Sujet: Ezrin is a member of the ERM (ezrin-radixin-moesin) family of proteins that function as crosslinkers between the actin cytoskeleton and the plasma membrane. Ezrin is a substrate for the tyrosine kinase epidermal (EGF) and hepatocyte growth factor (HGF) receptors. Upon EGF or HGF stimulation, ezrin is phosphorylated on tyrosine 353 (Y353), which is required for its interaction with phosphatidylinositol 3 kinase p85 subunit and activation of Akt. The I66-386 monoclonal antibody recognizes the phosphorylated Y353 of human ezrin.

Pathways: Maintenance of Protein Location