S6 (pSer240) anticorps (Alexa Fluor 488)

N° du produit ABIN1177168

Cet anticorps Souris Monoclonal détecte spécifiquement S6 dans ICS et BI. Il présente une réactivité avec des échantillons de Humain, Souris et Rat. Il a été cité dans 6+ publications.

Aperçu rapide pour S6 (pSer240) anticorps (Alexa Fluor 488) (ABIN1177168)

Antigène: S6

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Alexa Fluor 488

Application: Intracellular Staining (ICS), BioImaging (BI)

Clone: N4-41

Détail du produit

Épitope: pSer240

Marque: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human ribosomal protein S6 Peptide

Isotype: IgG1 kappa

Information d'application

Indications d'application: This antibody conjugate is suitable for intracellular staining of human peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Référence

Jastrzebski, Hannan, Tchoubrieva, Hannan, Pearson: "Coordinate regulation of ribosome biogenesis and function by the ribosomal protein S6 kinase, a key mediator of mTOR function." dans: Growth factors (Chur, Switzerland), Vol. 25, Issue 4, pp. 209-26, (2007) (PubMed).

Gu, Santiago, Mitchell: "Synergy between imatinib and mycophenolic acid in inducing apoptosis in cell lines expressing Bcr-Abl." dans: Blood, Vol. 105, Issue 8, pp. 3270-7, (2005) (PubMed).

Lal, Li, Smith, Sassano, Uddin, Parmar, Tallman, Minucci, Hay, Platanias: "Activation of the p70 S6 kinase by all-trans-retinoic acid in acute promyelocytic leukemia cells." dans: Blood, Vol. 105, Issue 4, pp. 1669-77, (2005) (PubMed).

Shah, Hunter: "Critical role of T-loop and H-motif phosphorylation in the regulation of S6 kinase 1 by the tuberous sclerosis complex." dans: The Journal of biological chemistry, Vol. 279, Issue 20, pp. 20816-23, (2004) (PubMed).

Shah, Ghosh, Hunter: "Mitotic regulation of ribosomal S6 kinase 1 involves Ser/Thr, Pro phosphorylation of consensus and non-consensus sites by Cdc2." dans: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 16433-42, (2003) (PubMed).

Kleijn, Proud: "The regulation of protein synthesis and translation factors by CD3 and CD28 in human primary T lymphocytes." dans: BMC biochemistry, Vol. 3, pp. 11, (2002) (PubMed).

Détail du antigène

Antigène: S6

Sujet: Ribosomal protein S6 (~29 kDa calculated and ~32 kDa observed molecular weights) is a component of the 40S ribosomal subunit and belongs to the S6E family of ribosomal proteins. The S6 ribosomal protein plays a role in regulating the translation of RNAs and thus controlling the growth and proliferation of cells. S6 ribosomal protein phosphorylation, especially at multiple C-terminal serine residues S235, S236, S240, and S244, activates S6. The activated S6 ribosomal protein in turn upregulates the ribosomal translation of RNA species coding for other ribosomal proteins, peptide elongation factors and other proteins involved in cell cycle entry and progression. These phosphorylations are mediated by various kinases (e.g., p70S6K and PKCD) activated through cellular responses to growth factors, cytokines, tumor promoting agents, and mitogens. The S6 ribosomal protein can be dephosphorylated in growth-arrested cells. The N4-41 monoclonal antibody specifically detects the S6 ribosomal protein phosphorylated at S240.
Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6