IL-10 anticorps

N° du produit ABIN1177280

L’anticorps Rat Monoclonal anti-IL-10 a été validé pour ELISA, Neut et ICS. Il convient pour détecter IL-10 dans des échantillons de Humain et Virus. Il y a 4+ publications disponibles.

Aperçu rapide pour IL-10 anticorps (ABIN1177280)

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivité: Humain, Virus

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-10 est non-conjugé

Application: ELISA, Neutralization (Neut), Intracellular Staining (ICS)

Clone: JES3-9D7

Détail du produit anti-IL-10 anticorps

Marque: BD Pharmingen™

Attributs du produit: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-10. Neutralization activity may be measured with a proliferation assay using 3 ng/mL recombinant human IL-10 (Cat.No. 554611), co-stimulated with 0.1 ng/mL recombinant mouse IL-4 (Cat.No. 550067), to stimulate MC/9 or D36 cells as indicator cells (i.e preincubation of the antibody with recombinant human IL-10 for 60 min)
50% Neutralization (ND50) at 0.2 - 4.0 ng/mL
>95% Neutralization at 3 - 30 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Stérilité: 0.2 μm filtered

niveau d'endotoxine: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogène: Recombinant Human IL-10

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Agent conservateur: Azide free

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Références pour anticorps anti-IL-10 (ABIN1177280)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." dans: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Burdin, Péronne, Banchereau, Rousset: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." dans: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, (1993) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." dans: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Gotlieb, Abrams, Watson, Velu, Berek, Martínez-Maza: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." dans: Cytokine, Vol. 4, Issue 5, pp. 385-90, (1992) (PubMed).

Détails sur IL-10

Antigène: IL-10 (IL10) (Interleukin 10 (IL10))

Autre désignation: IL-10

Sujet: The JES3-9D7 antibody reacts with human IL-10 and viral IL-10. The immunogen used to generate the JES3-9D7 hybridoma was recombinant human IL-10 expressed in COS cells.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints