IL-10 anticorps
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- Antigène Voir toutes IL-10 (IL10) Anticorps
- IL-10 (IL10) (Interleukin 10 (IL10))
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Reactivité
- Humain, Virus
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Hôte
- Rat
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp IL-10 est non-conjugé
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Application
- ELISA, Neutralization (Neut), Intracellular Staining (ICS)
- Marque
- BD Pharmingen™
- Attributs du produit
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-10. Neutralization activity may be measured with a proliferation assay using 3 ng/mL recombinant human IL-10 (Cat.No. 554611), co-stimulated with 0.1 ng/mL recombinant mouse IL-4 (Cat.No. 550067), to stimulate MC/9 or D36 cells as indicator cells (i.e preincubation of the antibody with recombinant human IL-10 for 60 min)
50% Neutralization (ND50) at 0.2 - 4.0 ng/mL
>95% Neutralization at 3 - 30 ng/mL - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Stérilité
- 0.2 μm filtered
- niveau d'endotoxine
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- Immunogène
- Recombinant Human IL-10
- Clone
- JES3-9D7
- Isotype
- IgG1
- Top Product
- Discover our top product IL10 Anticorps primaire
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- Agent conservateur
- Azide free
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." dans: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." dans: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, (1993) (PubMed).
: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." dans: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).
: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." dans: Cytokine, Vol. 4, Issue 5, pp. 385-90, (1992) (PubMed).
: "
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Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." dans: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).
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- Antigène
- IL-10 (IL10) (Interleukin 10 (IL10))
- Autre désignation
- IL-10 (IL10 Produits)
- Synonymes
- anticorps CSIF, anticorps GVHDS, anticorps IL-10, anticorps IL10A, anticorps TGIF, anticorps Il-10, anticorps IL10X, anticorps interleukin 10, anticorps IL10, anticorps Il10
- Sujet
- The JES3-9D7 antibody reacts with human IL-10 and viral IL-10. The immunogen used to generate the JES3-9D7 hybridoma was recombinant human IL-10 expressed in COS cells.
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
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