GFP anticorps (FITC)

N° du produit ABIN117893

L’anticorps anti-GFP Monoclonal Souris est utilisé pour la détection de GFP dans des échantillons de Aequorea victoria. Il a été validé pour IF.

Aperçu rapide pour GFP anticorps (FITC) (ABIN117893)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GFP est conjugé à/à la FITC

Application: Immunofluorescence (IF)

Clone: 9F9-F9

Détail du produit anti-GFP anticorps (FITC)

Specificité: This product was prepared from tissue culture supernatant by Protein A chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against wild type, recombinant and enhanced forms of GFP.

Attributs du produit: Absorption / Emission: 495 nm / 528 nm
Molar Ratio: 3.5 moles FITC per mole of Mouse IgG).

Purification: Protein A chromatography.

Immunogène: The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Isotype: IgG2a

Information d'application

Indications d'application: Designed to detect GFP and its variants in ELISA (sandwich or capture), Immunoblottingand Immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISAfor direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein

ID gène: 6100

UniProt: P42212