DNMT3L anticorps (AA 152-164)

N° du produit ABIN118012

Cet anticorps Lapin Polyclonal détecte spécifiquement DNMT3L dans WB, IP et EIA. Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour DNMT3L anticorps (AA 152-164) (ABIN118012)

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DNMT3L est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Détail du produit anti-DNMT3L anticorps

Épitope: AA 152-164

Séquence: Peptide: G-L-L-Q-R-R-R-K-W-R-S-Q-L Protein: 1 maaipaldpe aepsmdvilv gsselsssvs pgtgrdliay evkanqrnie diciccgslq 61 vhtqhplfeg gicapckdkf ldalflyddd gyqsycsicc sgetllicgn pdctrcycfe 121 cvdslvgpgt sgkvhamsnw vcylclpssr sgllqrrrkw rsqlkafydr esenplemfe 181 tvpvwrrqpv rvlslfedik keltslgfle sgsdpgqlkh vvdvtdtvrk dveewgpfdl 241 vygatpplgh tcdrppswyl fqfhrllqya rpkpgspgpf fwmfvdnlvl nkedldvasr 301 flemepvtip dvhggslqna vrvwsnipai rsrhwalvse eelsllaqnk qssklaakwp 361 tklvkncflp lreyfkyfst eltssl

Specificité: This antibody reacts with DNMT3L.

Réactivité croisée (Details): Species reactivity (expected):Mouse (76 %).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase

Immunogène: A synthetic peptide corresponding to aa 152-164 of Human DNMT3L.

Information d'application

Indications d'application: This product has been assayed by ELISA, Western blotting and Immunoprecipitation. The antibody was tested by ELISA against 0.1 μg of the immunizing peptide. A1/1,000-1/3,000 dilution of the antibody is recommended for this assay. For Western blotting, first Immunoprecipitate (1/100 dilution) the antigen using this

Restrictions: For Research Use only

Stockage

Concentration: 1.43 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Shelf live: One year from despatch.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Should this product contain a precipitate we recommend microcentrifugation before use.

Détails sur DNMT3L

Antigène: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Autre désignation: DNMT3L

Sujet: DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.Synonyms: DNA (cytosine-5)-methyltransferase 3-like

ID gène: 29947

NCBI Accession: NP_037501

UniProt: Q9UJW3