ING5 anticorps (AA 127-140)

N° du produit ABIN118053

Cet anticorps anti-ING5 Polyclonal Chèvre (ABIN118053) détecte spécifiquement ING5 dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain et Singe.

Aperçu rapide pour ING5 anticorps (AA 127-140) (ABIN118053)

Antigène: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Reactivité: Humain, Singe

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ING5 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-ING5 anticorps

Épitope: AA 127-140

Séquence: Protein Sequence: Human p28 ING5 isoform 1, 240 aa, predicted MW 27.8 kDa: 1 matamylehy ldsienlpce lqrnfqlmre ldqrtedkka eidilaaeyi stvktlspdq 61 rverlqkiqn ayskckeysd dkvqlamqty emvdkhirrl dadlarfead lkdkmegsdf 121 essggrglkk grgqkekrgs rgrgrrtsee dtpkkkkhkg gseftdtils vhpsdvldmp 181 vdpneptycl chqvsygemi gcdnpdcpie wfhfacvdlt kpkgkwfcp rcvqekrkkk Protein Sequence: Human p28 ING5 isoform 2, 226 aa, predicted MW 25.9 kDa 1 matamylehy ldsienlpce lqrnfqlmre ldqrtedkka eidilaaeyi stvktlspdq 61 verlqkiqn ayskckeysd dkvqlamqty emvdkhirrl dadlarfead lkdkmegsdf 121 essggrglkk grgqkekrgs rgrgrrtsee dtpkkkkhkg gseftdtils vhpsdvldmp 181 vdpneptycl chqvsygemi gcdnpdcpie wfhfacvdlt tkpkgk

Specificité: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2 (see below). However, 100 % homology is on record for this protein from human and chimpanzee. Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.

Purification: Immunoaffinity chromatography.

Immunogène: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence shows homology to isoforms 1 and 2 for ING5.

Information d'application

Indications d'application: This affinity purified antibody has been tested for use in ELISA (1: 4,000 - 1: 16,000) and byWestern blot (1: 500-1: 1,000, Expect a band approximately 28 kDa or 26 kDa in sizecorresponding to variants of ING5 in the appropriate cell lysate or extract). Also suitable for Immunofluorescence microscopy.

Restrictions: For Research Use only

Stockage

Concentration: 1.2 mg/mL (UVabs. 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Détails sur ING5

Antigène: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Autre désignation: ING5

Sujet: ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. ING5 is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 acts as a tumor suppressor and is a potent growth regulator in normal and in established cells. ING5 may have involvement in chromatin remodeling.Synonyms: Inhibitor of growth protein 5, p28ING5

ID gène: 84289

NCBI Accession: NP_115705

UniProt: Q8WYH8

Pathways: Chromatin Binding