Biotin anticorps (HRP)

N° du produit ABIN118070

L’anticorps Chèvre Polyclonal anti-Biotin a été validé pour WB, IHC (p), EIA, IP et IHC (fro). Il convient pour détecter Biotin dans des échantillons de .

Aperçu rapide pour Biotin anticorps (HRP) (ABIN118070)

Antigène: Biotin

Reactivité: Veuillez nous consulter SVP

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Biotin anticorps (HRP)

Purification: Immunoaffinity chromatography

Immunogène: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Information d'application

Indications d'application: Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy andimmunohistochemistry as well as other peroxidase-antibody based enzymatic assaysrequiring lot-to-lot consistency. Recommended dilutions: Western blot 1: 500 - 1: 3,000. ELISA 1: 5,000 - 1: 25,000. Immunohistochemistry 1: 300 - 1: 1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL of deionized water or equivalent.

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Gentamicin Sulfate, 10 mg/mL BSA (IgG and Protease free)

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Conseil sur la manipulation: Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.