Biotin anticorps (Texas Red (TR))

N° du produit ABIN118072

Cet anticorps Chèvre Polyclonal détecte spécifiquement Biotin dans WB, FACS, IHC (p), IP, EIA et IHC (fro). Il présente une réactivité envers .

Aperçu rapide pour Biotin anticorps (Texas Red (TR)) (ABIN118072)

Antigène: Biotin

Reactivité: Veuillez nous consulter SVP

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la Texas Red (TR)

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Biotin anticorps (Texas Red (TR))

Attributs du produit: Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 4.8 Moles Texas RedTM per mole of Goat IgG F(ab')2.

Purification: Immunoaffinity chromatography

Immunogène: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Information d'application

Indications d'application: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Flow cytometry: 1.2,000 - 1: 10,000. IF microscopy: 1: 500 - 1: 2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.