CD8 anticorps (FITC)
Aperçu rapide pour CD8 anticorps (FITC) (ABIN118129)
Antigène
Voir toutes CD8 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
-
-
Specificité
- This antibody reacts with a non-polymorphic epitope on the CD8 alpha chain. Stains mouse Ly2.1 and Ly2.2 cells.
-
Purification
- Affinity chromatography on Protein A
-
Immunogène
- T cell clone, C6. Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
-
Isotype
- IgG2a
-
-
-
-
Indications d'application
-
Flow cytometry: Neat - 1/10, use 10 μL of the suggested working dilution to label 10^6 cellsin 100 μL, The Fc region of monoclonal antibodies may bind non-specifically to cellsexpressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
-
-
-
Concentration
- 0.1 mg/mL
-
Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
-
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Conseil sur la manipulation
- Avoid repeated freezing and thawing. Shelf live: one year from despatch.
-
Stock
- 4 °C/-20 °C
-
Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
-
- CD8
-
Autre désignation
- CD8
-
Sujet
- The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2
-
ID gène
- 12525
-
NCBI Accession
- NP_001074579
-
UniProt
- P01731
Antigène
-