Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox)
Specificité
This antibody (clone PK136) recognises the NK1.1 cell surface antigen. Although previously thought to recognise only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory. This antibody is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. The antibody has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
Réactivité croisée (Details)
Species reactivity (tested):Mouse.
Purification
Affinity Chromatography on Protein A
Immunogène
Spleen and bone marrow cells from CE mice. Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0 - Ag14 myeloma cell line.
KLRB1
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow Cytometry: use 10 μL of 5-10 μg/mL diluted antibody to label 1 x 10^6 cells in 100 μL. Immunoprecipitation. Cytotoxic Assays: 1-10 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
NK1.1 is a cell surface glycoprotein encoded by members of the NKR-P1 gene family. In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.Synonyms: C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, NKRP1A, Natural killer cell surface protein P1A