MHC Class II RT-1B anticorps
Aperçu rapide pour MHC Class II RT-1B anticorps (ABIN118332)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
-
-
Purification
- Protein G affinity chromatography
-
Immunogène
- Rat thymocyte membrane glycoproteins
-
Isotype
- IgG1
-
-
-
-
Indications d'application
-
Flow cytometry (1:50 - 1:100): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. This product is routinely tested in flow cytometry on rat splenocytes. Immunohistochemistry on frozen tissues and paraffin embedded sections (1:50 - 1:100): this clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde) (4). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
-
-
-
Concentration
- 1.0 mg/mL
-
Buffer
- PBS, pH 7.2, containing 0.09 % sodium azide
-
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Conseil sur la manipulation
- Avoid repeated freezing and thawing.
-
Stock
- -20 °C
-
-
- MHC Class II RT-1B
-
Autre désignation
- MHC Class II RT1B
-
Sujet
- Synonyms: HLA Class II, MHC Class 2 RT1B
Antigène
-