MHC Class II RT-1B anticorps

N° du produit ABIN118334

L’anticorps anti-MHC Class II RT-1B Monoclonal Souris est utilisé pour la détection de MHC Class II RT-1B dans des échantillons de Rat. Il a été validé pour FACS, IHC (fro), IHC (p) et WB.

Aperçu rapide pour MHC Class II RT-1B anticorps (ABIN118334)

Antigène: MHC Class II RT-1B

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MHC Class II RT-1B est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: OX-6

Détail du produit anti-MHC Class II RT-1B anticorps

Purification: Affinity Chromatography on Protein G

Immunogène: Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemsitry on paraffin sections: 1/50 - 1/100, This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6, This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur MHC Class II RT-1B

Antigène: MHC Class II RT-1B

Autre désignation: MHC Class II RT1B

Sujet: Synonyms: HLA Class II, MHC Class 2 RT1B