CD15 anticorps (Biotin)

N° du produit ABIN118585

Cet anticorps Souris Monoclonal détecte spécifiquement CD15 dans FACS. Il présente une réactivité envers Humain.

Aperçu rapide pour CD15 anticorps (Biotin) (ABIN118585)

Antigène: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD15 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: TG-1

Détail du produit anti-CD15 anticorps (Biotin)

Purification: Ion exchange chromatography and gel filtration

Immunogène: Thymocyte glycoprotein fraction adsorbed to Con A and eluted with 0.1 M glucose

Isotype: IgM

Information d'application

Indications d'application: In flow cytometry (1.0 x 10^6 cells ), IFA and IHC (acetone-fixed frozen sections). May be used for enumerating mature granulocytes in peripheral blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 100 µg/mL (OD280nm)

Buffer: 0.01 M PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CD15

Antigène: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Autre désignation: CD15

Sujet: The CD15 antigen is expressed on approximately 90 % human circulating granulocytes (membranes and granules), 30 to 60 % of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.Synonyms: Lacto-N-Fucopentaose III, Lewis X, SSEA1, Stage-Specific Embryonic Antigen, X-Hapten