This antibody reacts with the Alpha 5 integrin. Has been shown to stimulate homotypic cellular aggregation of alpha 5 beta 1 positive lymphoid cell lines. It also inhibits the adherence of cells to fibronectin.
Purification
Affinity Chromatography on Protein G
Immunogène
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
ITGA5
Reactivité: Humain
WB, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Immunoprecipitation: 1-5 μg/mL. Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 1x10^6 cells in 100 μL of whole blood. Immunohistochemistry on Frozen Sections: 0.5-1 μg/mL. Recommended Positive Control: Human Endothelium. Functional assays: Removal of Sodium Azide is recommended prior to use. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD49e is the integrin alpha 5 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 5 undergoes post translational cleavage in the extracellular domain to yield disulfide linked light and heavy chains that join with beta 1 to form a fibronectin receptor that is known variously: in lymphocytes as very late (activation) antigen 5 (VLA5), in platelets as glycoprotein IcIIa, and in fibroblasts as extracellular matrix receptor 6 (ECMVI). In addition to adhesion, integrins are known to participate in cell surface mediated signalling.Synonyms: CD49 antigen-like family member E, FNRA, Fibronectin receptor subunit alpha, Integrin alpha-5, Integrin alpha-F, VLA-5, VLA5