CD58 anticorps

N° du produit ABIN118746

Cet anticorps anti-CD58 est un anticorps Souris Monoclonal détectant CD58 dans FACS et WB. Adapté pour Humain.

Aperçu rapide pour CD58 anticorps (ABIN118746)

Antigène: CD58

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD58 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB)

Clone: BRIC-5

Détail du produit anti-CD58 anticorps

Specificité: BRIC5 was produced in response to erythrocytes. It reacts by Immunoblotting to non-reduced erythrocyte membranes. BRIC5 is an indirect haemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC5 inhibits T cell rosetting.Aff. Const.:4 x 10e8 M-1

Purification: Affinity Chromatography on Protein G.

Immunogène: Human erythrocytes.

Isotype: IgG2a

Information d'application

Indications d'application: Flow Cytometry: Use 10 μL of 1/10 diluted antibody to label 10^6 cells in 100 μL. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CD58

Antigène: CD58

Autre désignation: CD58 / LFA3

Sujet: CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5,000 CD58 Molecules on each erythrocyte. There is reduced expression of CD58 on haemopoietic cells in individuals with paroxysmal nocturnal haemoglobinuria.Synonyms: Ag3, LFA-3, Lymphocyte function-associated antigen 3

ID gène: 965

NCBI Accession: NP_001138294

UniProt: P19256