L-Selectin anticorps

N° du produit ABIN118763

L’anticorps Souris Monoclonal anti-L-Selectin a été validé pour FACS, IP et IHC (fro). Il convient pour détecter L-Selectin dans des échantillons de Chemical.

Aperçu rapide pour L-Selectin anticorps (ABIN118763)

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Reactivité: Chemical

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp L-Selectin est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: FMC46

Détail du produit anti-L-Selectin anticorps

Specificité: Antibody SM1149PS recognizes CD62L, also known a L-Selectin.

Purification: Affinity Chromatography on Protein G

Immunogène: PHA stimulated lymphoblasts

Isotype: IgG2b

Information d'application

Indications d'application: Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunoprecipitation. Immunohistochemistry on frozen sections. Recommended positive control tissue: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the undiluted antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur L-Selectin

Antigène: L-Selectin (SELL) (Selectin L (SELL))

Autre désignation: CD62L / L-Selectin

Classe de substances: Chemical

Sujet: CD62L is a 74-95 kDa member of the selectin family of adhesion receptors, which acts as a ligand for both CD62-P (P-selectin) and CD62E (E-selectin). Human CD62L is constitutively expressed on most leucocytes including monocytes, granulocytes, lymphocytes, NK cells, bone marrow myeloid progenitor cells and on a subset of thymocytes. CD62L plays an important role in leucocyte tethering and rolling on the endothelial cell surface and for the homing of naive lymphocytes to lymph nodes and Peyers patches via HEV. Neutrophils require a constant supply of this molecule on the cell surface for migration into peripheral tissues and adhesion to activated endothelium at sites of inflammation, where CD62L is rapidly shed as soluble L-selectin, but surface expression still remains. The expression of CD62L is down regulated on lymphocytes and neutrophils by PMA stimulation.Synonyms: CD62 antigen-like family member L, LAM-1, LECAM1, LNHR, LYAM1, Leu-8, Leukocyte adhesion molecule 1, Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1, Lymph node homing receptor, SELL, TQ1, gp90-MEL

ID gène: 6402

NCBI Accession: NP_000646

UniProt: P14151